The S.pneumoStrip kit is the only commercial molecular test that allows rapid, simple and reliable detection of 76 serotypes of Streptococcus pneumoniae (S.pneumoniae), including the 23 serotypes present in current pneumococcal vaccines (7-valent, 10-valent, 13-valent and 23-valent). Thanks to its technology optimised for the use of direct sampling, without the need for prior positive culture, it offers an accurate and accessible diagnosis, facilitating decision making in laboratories, hospitals and research centres.
Infections caused by S. pneumoniae are a major public health problem worldwide, with high morbidity and mortality in both industrialised and resource-poor countries. This pathogen is responsible for diseases ranging from otitis media and acute sinusitis to severe invasive infections such as pneumonia, meningitis and septicaemia, causing 1.6 million deaths annually, 800,000 of them in children under 5 years of age. The World Health Organisation (WHO) recommends epidemiological surveillance to assess the impact of vaccination strategies and to monitor the circulation of different pneumococcal serotypes. Traditionally, this serotyping has been performed using the Quellung technique, which is recognised as the reference method, but requires time, specific reagents and highly trained personnel.
The S.pneumoStrip kit revolutionises this process by providing fast and accurate results, without the need for laborious techniques or highly specialised equipment, making it an essential tool for the diagnosis and investigation of pneumococcal disease.
Only commercial molecular test
Perfect correlation with Gold Standard
76 serotypes in one test
Possibility to automate the hybridization process
No need of previous culture
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C1
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Utility of a Reverse-Hybridization Strip Assay for Streptococcus pneumoniae serotyping
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English
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C2
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Streptococcus pneumoniae detection based in the cpsA and cpsC genes will miss serotypes 25A, 25F and 38
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English
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C3
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Utility of PneumoStrip, a Reverse-Hybridization Nylon Strip Assay prototype, for Streptococcus pneumoniae serotyping
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English
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C4
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Direct-serotyping-of-streptococcus-pneumoniae-in-blood-culture-samples-by-using-a-PCR-reverse-hybridization-test
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English
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C5
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Direct-identification-of-Streptococcus-pneumoniae-serotypes-in-cultures-negative-samples-by-a-PCR-reverse-hybridization-technique
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English
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C6
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Impacto de la vacunada conjugada 13-valente en la enfermedad neumocócica invasiva en Navarra
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Spanish
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C7
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Direct identification of Streptococcus pneumoniae serotypes in culture negative paediatric clinical samples by a PCR reverse-hybridization strip-based commercial assay
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English
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A1
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A reverse-hybridization test for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs
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English
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A2
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Direct identification of pneumococal serotypes in blood cultures by a PCR-reverse-hybridization technique
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English
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A3
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Comparison of Four Methods for Streptococcus pneumoniae capsular typing
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English
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A4
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Carriage and distribution of serotypes Streptococcus pneumoniae in healthy vaccinated children for the year 2021 in Bulgaria
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English
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A5
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High frequency of Streptococcus pneumoniaeserotype 3 in negative pleural fluid culturesfrom paediatric samples obtained in the Madridregion from 2018 to 2022, detected by directidentification using PCR-reverse-hybridizationstrip-based assay
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English
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A6
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Impact of the progressive uptake of pneumococcal conjugate vaccines on the epidemiology and antimicrobial resistance of invasive pneumococcal disease in Gipuzkoa, northern Spain, 1998-2022
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English
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A7
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Predominant Pneumococcal Serotypes in Isolates Causing Invasive Disease in a Spanish Region: An Examination of Their Association with Clinical Factors, Antimicrobial Resistance, and Vaccination Coverage
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English
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A8
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Impact of the National Vaccination Strategy on the Prevalence of Streptococcus pneumoniae and Its Serotypes Among Clinically Healthy Children Under Six Years of Age During and After the COVID-19 Pandemic
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English
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A9
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Comparative Analysis of Pneumococcal Serotypes for 10 Years (2014-2024) in the Comunidad Valenciana Region, Spain, and How They Are Correlated with PCV13, PCV20, and PCV21
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English
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A10
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Respiratory infections in the post-COVID-19 era: impact, prevalence, and clinical characteristics of bacterial and viral co-infections
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English
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A11
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Pneumococcal Meningitis in a Region of Northern Spain, 1993-2023: Incidence Trends, Clinical Features, Recurrences, and Antibiotic Resistance
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English
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The kit was initially designed to be used on culture samples, so the best results are obtained using them. Nevertheless, it has been shown in different studies to be useful also with non-cultured samples. In those cases, the sensitivity of the test may be affected by having less DNA in the starting sample, which would be reflected in the lack of signal in the internal controls of the test. If so, sensitivity can be increased by performing individual PCRs with each set of primers, instead of performing a single PCR with all primers.
No. The detection of 3 internal controls allows unequivocal identification of S. pneumoniae, even in the presence of other pneumococci.
