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Hybridization on strip and real-time PCR (rt PCR) products.
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MOLECULAR DIAGNOSTICS

Molecular diagnostic tests are used to detect specific DNA or RNA sequences associated with several health problems.

These tests are based on the amplification of nucleic acids by polymerase chain reaction (PCR), as well as the subsequent hybridization of these fragments with specific probes for the target to be detected. This hybridization can be carried out using different techniques.

In the reverse blot technique, hybridization is carried out with probes immobilised on a membrane after PCR has been completed. In this way, up to 24 targets can be detected in a single test, facilitating diagnosis in case of co-infections or when there are different markers for the same genetic condition. This technique is used by the products of the Opegen line.

In the real time PCR technique, on the other hand, hybridization occurs with fluorophore-labelled probes during the PCR process. This allows up to four targets to be detected in a single test, quickly and easily. This technique is used for the products of the rt PCR line.

How are our tests?

Features

Products based on reverse blot or hybridization on strip allow the detection of up to 24 targets in a single test, obtaining sensitive, specific and reliable results. The hybridization process can be automated, and the reading of results can be done visually or with the help of a reader that runs a programme developed specifically for each product.

Real time PCR-based products allow the detection of up to 4 targets in a fast, simple, sensitive and specific test, which makes them very useful when a rapid diagnosis is needed.

All our kits can be transported in refrigerated conditions, avoiding the use of dry ice and all the logistical and economic complications linked to it.

All our kits have all the components needed ready to use, avoiding manipulations and facilitating the process.

All our kits use an internal endogenous control to ensure that the whole process has been carried out correctly (extraction, retrotranscription if applicable, amplification, detection).

Some of our kits allow for a short lysis process that replaces the nucleic acid extraction process, thus facilitating and reducing the cost of the procedure.

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information

    Molecular tests for the detection of 14 bacteria associated with respiratory tract infections

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    PCR procedure
    Hybridization process: TENDIGO
    Hybridization process: PROFIBLOT
    Hybridization process: PST-60HL
    Animated conventional PCR procedure

    FAQ

    For hybridization on strip tests, a conventional thermal cycler and a thermal shaker are required. For real time PCR tests, a real time thermal cycler is required. The validated devices are indicated in the instructions of each product, but other devices could be used if the user validates their use.

    No, extraction reagents are not included in the kit. Some of our kits allow a short lysis process that replaces the nucleic acid extraction process, facilitating and reducing the costs of the procedure. These lysis reagents are included in these kits.

    All our kits have all the needed components already prepared for use, avoiding manipulations and facilitating the process.

    All hybridization on strip products have the same hybridization protocol, and the PCR protocols are very similar. For real time PCR products, all products belonging to the same line have the same PCR protocol.

    PCR or polymerase chain reaction is a process that allows the targeted amplification of specific areas of DNA in such a way that, starting from very small amounts of material, a large number of copies of the desired area can be obtained. It is used for numerous applications, including the detection of biological material from different organisms, the detection of mutations leading to genetic diseases, the analysis of polymorphisms (population studies, paternity studies), etc. How to process the PCR product depends on what is to be analysed. If you only want to see if amplification has occurred, gel electrophoresis is the most common method. If you want to detect a specific sequence, there are multiple options: restriction analysis (digestion with restriction enzymes and visualisation of the products obtained), blot (hybridisation on nylon membranes with specific probes), ELISA, sequencing, etc.