Molecular diagnostic tests are used to detect specific DNA or RNA sequences associated with several health problems.
These tests are based on the amplification of nucleic acids by polymerase chain reaction (PCR), as well as the subsequent hybridization of these fragments with specific probes for the target to be detected. This hybridization can be carried out using different techniques.
In the reverse blot technique, hybridization is carried out with probes immobilised on a membrane after PCR has been completed. In this way, up to 24 targets can be detected in a single test, facilitating diagnosis in case of co-infections or when there are different markers for the same genetic condition. This technique is used by the products of the Opegen line.
In the real time PCR technique, on the other hand, hybridization occurs with fluorophore-labelled probes during the PCR process. This allows up to four targets to be detected in a single test, quickly and easily. This technique is used for the products of the rt PCR line.
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No, extraction reagents are not included in the kit. Some of our kits allow a short lysis process that replaces the nucleic acid extraction process, facilitating and reducing the costs of the procedure. These lysis reagents are included in these kits.
All our kits have all the needed components already prepared for use, avoiding manipulations and facilitating the process.
PCR or polymerase chain reaction is a process that allows the targeted amplification of specific areas of DNA in such a way that, starting from very small amounts of material, a large number of copies of the desired area can be obtained. It is used for numerous applications, including the detection of biological material from different organisms, the detection of mutations leading to genetic diseases, the analysis of polymorphisms (population studies, paternity studies), etc. How to process the PCR product depends on what is to be analysed. If you only want to see if amplification has occurred, gel electrophoresis is the most common method. If you want to detect a specific sequence, there are multiple options: restriction analysis (digestion with restriction enzymes and visualisation of the products obtained), blot (hybridisation on nylon membranes with specific probes), ELISA, sequencing, etc.