Search

Test for the determination of the HLA-B27 alelles

HLA-B27 kit is a test based on the reverse hybridization principle and allows the determination of the HLA-B27 alleles, associated to several autoimmune pathologies as ankylosing spondylitis, acute anterior uveitis, reactive arthritis and inflammatory bowel disease.

The Major Histocompatibility System comprises a set of genes known in humans as HLA system. The proteins encoded by those genes are called “HLA molecules” or “HLA antigens”.

The existence of a genetic predisposition in individuals carrying the HLA-B*27 histocompatibility antigen has been demonstrated for the following diseases:

  • Ankylosing spondylitis: is a chronic, disabling rheumatic disease, that usually affects males between 20 and 30 years.
  • Uveitis: an inflammation of the uveal tract.
  • Reactive arthritis: an inflammation of one or more joints that appears as a reaction to an infection.
  • Inflammatory bowel disease: refers to a group of disorders that predominantly affect the intestine and which are characterized by chronic inflammation, as Crohn’s disease or ulcerative colitis.

HLA-B27 test is a simple and reliable tool for helping professionals in the diagnosis of the pathologies previously mentioned.

search 1

Detection of all the HLA B27 alleles

prueba especifica sensible

Specific and sensitive

lectura mediante scanner

Automated reading with scanner available

automatiza proceso hibridacion

Possibility to automate the hybridization process

Our products for the determination of the HLA-B27 alelles

Product
Technique
Sample
Promotional Sheet
CE Decl.
Instruct.
Catalogue No.
HLA B27 Strip
Hybridization on Strip
DNA extracted from blood, saliva or tissues
3.154.016.53.000

All the HLA-B27 are detected, related to the probability of developing certain autoimmune diseases as ankylosing spondylitis, acute anterior uveitis, reactive arthritis and inflammatory bowel disease.

As it detects the genes associated with the alleles, it is a specific and highly sensitive test that eliminates the problems of cross-reactivity that can occur with other tests, such as flow cytometry.

The interpretation of the test is very simple, as it is performed visually with the help of a control strip template. In addition, a scanner with a specific program designed for the product can be used to facilitate reading and storing the results.

The hybridization process can be performed manually or using an automated system, so that the user only has to set up the reagents and strips, and remove the developed strips once the process is finished. This reduces the time that the user has to dedicate to the process.

Contact us for more
information

    Frequently Asked Questions

    No. According to the allele sequence review, there is no cross-reactivity with them.

    For the PCR amplification step: thermocycler (PTC-100 (MJ Research), MJ Mini Gradient Thermal Cycler (BioRad), Mastercycler Personal (Eppendorf), S1000 Thermal Cycler (BioRad)). For the hybridization step: thermoshaker (Manual protocol: PST-60HL (Biosan S.L.)). → 16 samples. Automated protocol: Profiblot T30 (Tecan) → 30 samples. Profiblot T48 (Tecan) → 48 samples. Autoblot 3000H (medTEC) → 20 samples.TENDIGO (Fujirebio) → 10 samples. DynaHeat (Dynex) → 30 samples. For the results interpretation step: strip template or reader: BloTrix R2 (BioSciTEc) → 16 samples. BloTrix S1 → 48 samples.

    For the PCR amplification step: any thermocycler can be used, as long as the ration volume is 50 µl. For the hybridization step: any thermocycler can be used, but a perfect temperature control must be ensured, as this step is critic. For the results interpretation step: no device is needed, it is only an aid. If needed, only Blotrix devices can be used, as no other devices are validated.