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HLA-B27 kit is a test based on the reverse hybridization principle and allows the determination of the HLA-B27 alleles, associated to several autoimmune pathologies as ankylosing spondylitis, acute anterior uveitis, reactive arthritis and inflammatory bowel disease.
The Major Histocompatibility System comprises a set of genes known in humans as HLA system. The proteins encoded by those genes are called “HLA molecules” or “HLA antigens”.
The existence of a genetic predisposition in individuals carrying the HLA-B*27 histocompatibility antigen has been demonstrated for the following diseases:
HLA-B27 test is a simple and reliable tool for helping professionals in the diagnosis of the pathologies previously mentioned.
Detection of all the HLA B27 alleles
Specific and sensitive
Automated reading with scanner available
Possibility to automate the hybridization process
No. According to the allele sequence review, there is no cross-reactivity with them.
For the PCR amplification step: thermocycler (PTC-100 (MJ Research), MJ Mini Gradient Thermal Cycler (BioRad), Mastercycler Personal (Eppendorf), S1000 Thermal Cycler (BioRad)). For the hybridization step: thermoshaker (Manual protocol: PST-60HL (Biosan S.L.)). → 16 samples. Automated protocol: Profiblot T30 (Tecan) → 30 samples. Profiblot T48 (Tecan) → 48 samples. Autoblot 3000H (medTEC) → 20 samples.TENDIGO (Fujirebio) → 10 samples. DynaHeat (Dynex) → 30 samples. For the results interpretation step: strip template or reader: BloTrix R2 (BioSciTEc) → 16 samples. BloTrix S1 → 48 samples.
For the PCR amplification step: any thermocycler can be used, as long as the ration volume is 50 µl. For the hybridization step: any thermocycler can be used, but a perfect temperature control must be ensured, as this step is critic. For the results interpretation step: no device is needed, it is only an aid. If needed, only Blotrix devices can be used, as no other devices are validated.
