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One-step immunochromatographic tests for the differential detection of Glutamate Dehydrogenase (GDH) as well as C. difficile toxins A and B in human faeces
C. difficile is a bacteria considered to be responsible for approximately 25% of the diarrhoea associated with the consumption of antibiotics. In addition to these symptoms, the disease can lead to other complications (such as pseudomembranous colitis – PMC) that require urgent antibiotic treatment, as they can be life-threatening. In fact, mortality associated with C. difficile can reach up to 30%.
GDH is an enzyme produced in large quantities by C. difficile strains, which makes it an excellent marker for determining infections caused by this micro-organism. In addition, C. difficile can release toxins (A and/or B), which are the main virulence factors responsible for causing clinical symptoms.
In the diagnosis of C. difficile infection, it is recommended to test all samples for GDH antigen if the origin of the diarrhoea is unknown. In case of a positive GDH antigen test, a second test for toxin A and/or B is recommended.
The Simple/Stick GDH, Simple/Stick 2A-Bdiff and Simple GDH-Toxins tests allow this protocol to be followed, either with a single test or with two independent tests.
≤15 minutes
Complete diagnostic protocol in a single test
Simple and safe
Possibility of automated reading
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Product
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Catalogue No.
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Contents
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Sample
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Promotional Sheet
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CE Decl.
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Instruct.
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Simple/Stick GDH
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9.045.020.00.000
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Simple 20 tests/kit. Visual or automated reading
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Stool
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9.045.020.71.000
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Simple 20 tests/kit. Visual reading
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9.045.005.00.000
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Simple 5 tests/kit. Visual or automated reading
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Simple/Stick 2A-Bdiff
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9.038.020.00.000
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Simple 20 tests/kit. Visual or automated reading
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Stool
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9.038.005.00.000
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Simple 5 tests/kit. Visual or automated reading
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Simple GDH-Toxins
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9.065.020.20.000
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Simple 20 tests/kit. Visual or automated reading
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Stool
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Positive control (GDH-Toxins)
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9.065.011.12.000
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1 swab/kit
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N/A
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N/A
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Simple/Stick GDH, Simple/Stick 2A-Bdiff and Simple GDH-Toxins are all qualitative. This means that the test shows a positive or negative result for the detection of both GDH and toxins A and B, but does not quantify the amount of the positive.
The tests are manufactured with a shelf life of 24 months.
The Simple/Stick GDH and Simple/Stick 2A-Bdiff tests are available in either Simple format (i.e. cassette included) or Stick format (reaction strips only).
Depending on the reference, we can find from kits that include 5 tests to kits that include 20.
The Simple/Stick 2A-Bdiff and Simple GDH-Toxins tests give results within 15 minutes, while the Simple/Stick GDH test gives results within 10 minutes.
As indicated in the instructions for use, the test was designed for detection of C.difficile in fresh faeces.
It is recommended to analyse fresh, untreated samples. If they have to be stored for a period of time, they can be kept in the refrigerator (+2-8ºC) for 1 or 2 days. For longer periods, they should be frozen at -20°C, taking into account that some samples lose immunoreactivity after freezing.
They can be done both visually and automated, through the OPERON Strip Reader.
No, all products are transported at room temperature, so handling is simple and economical.
The kits can be stored at room temperature.
