One-step immunochromatographic test for the qualitative detection, on separate strips, of Cryptosporidium sp, Giardia lamblia and Entamoeba histolytica in human stools.
Cryptosporidium sp, Giardia lamblia and Entamoeba histolytica are three of the main groups of protozoa causing gastrointestinal infections.
Cryptosporidiosis is a protozoan infection caused by Cryptosporidium sp in humans. It is usually an acute infection of short duration. However, in children or immunocompetent patients, symptoms may be more severe.
Giardia lamblia is the parasite that causes Giardiasis in humans. This organism is usually responsible for 25% of gastrointestinal diseases, although it can also occur asymptomatically.
Entamoeba histolytica is the causative agent of dysentery and amoebiasis. This parasitic disease is the third leading cause of human mortality after malaria and schistosomiasis, and is estimated to be responsible for 50,000-100,000 deaths each year.
The traditional diagnostic method consists of microscopic examination of faeces, which requires experienced personnel for recognition. For this reason, the development of an alternative, rapid and simple method that does not require the presence of the intact organism for recognition is of interest.
Rapid tests for the detection of the antigen of the three protozoa are a perfect alternative to make a first approach to the diagnosis of any of these pathologies.
3 parasites in 1 test
Simple and safe
Automated reading possible
Theoretically, the antibodies we use to detect Giardia lamblia recognise a 65 kDa protein complex that is part of the cysto wall (Giardia’s form of resistance and propagation). This complex is composed of CWP1 (26kDa) and CWP2 (39kDa) proteins that bind shortly after their synthesis to form the 65 kDa complex within ESVs (specific encystment vesicles). In this process CWP2 (39kDa) is processed into a 26kDa protein, so that both species coexist in ESVs, although only the processed 26 kDa form will exist in the cyst wall.”
It is not recommended to use samples with preservation agents (such as formalin, PBS, PVA or similar) or enrichment media, as their presence could interfere with the correct performance of the test.
Samples can be stored in the refrigerator (approximately 4°C) for 1-2 days before testing. For longer storage, they should be stored in the freezer at -20°C without prior handling.