One-step immunochromatographic tests for the detection of soluble Streptococcus pneumoniae antigen and Legionella pneumophila serogroup 1 in human urine.
Streptococcus pneumoniae is the main pathogen responsible for pneumonia, whether community-acquired or hospital-acquired (nosocomial), in adults and children. Twenty-five percent of pneumonia caused by pneumococcus pneumoniae are bacteremic, i.e. the bacteria can be detected in blood, which is associated with higher mortality. However, many cases of non-bacteremic pneumococcal pneumonia are not detected by traditional methods of culture of respiratory or blood samples.
Legionella pneumophila is the species that causes more than 90% of Legionella infections in humans. In particular, Legionella pneumophila serogroup 1 is the most frequently isolated serogroup and represents more than 80% of clinically diagnosed cases. Legionella infection or legionellosis can have two distinct clinical forms: pulmonary infection or “Legionnaires’ disease”, which is characterised by pneumonia with high fever, and the non-pneumonic form known as “Pontiac fever”, which does not normally require treatment.
The Simple/Stick Legio pneumo, Simple/Stick Strep pneumo and Simple/Stick Strep pneumo-Legio tests allow the detection and identification of soluble S. pneumoniae and L. pneumophila antigens excreted in urine, enabling a rapid and reliable diagnosis in the early stages of the disease.
Simple and safe
As indicated in the instructions for use of the tests, after dilution of the sample in the buffer included in the test, it is only necessary to add a certain volume of the diluted sample to the test strip and wait for 20 minutes.
Detects Legionella pneumophila serogroup 1, responsible for 80% of cases clinically diagnosed as Legionella infection or legionellosis.
Boric acid can be used as a preservative up to a concentration of 20 mg/ml, as indicated in the instructions for use of the product.
Samples can be kept at room temperature (+15-30°C) if the test is to be performed within 24 hours of sample collection, or they can be kept in the refrigerator for 1-2 days. For longer storage, they should be frozen at -20°C.