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Test for the genotyping of polymorphisms associated with lactase persistence

LactoStrip kit is a test based on the reverse hybridization principle and allows the detection of two polymorphisms of MCM6 gene associated to the lactase persistance in adults: C/T 13910 and G/A 22018.

In most humans, the ability to digest the lactose (the main carbohydrate present in the milk) quickly decreases after the end of lactation period (primary lactose intolerance). This is due to a reduction of the enzyme lactase–phlorizin hydrolase (LPH), responsible for the hydrolysis of lactose to glucose and galactose, sugars that are easily absorbed in the intestine. However, certain individuals, mainly descendants of traditionally cattle-raising peoples, retain the ability to digest lactose in adulthood.

Lactose intolerance presents with diverse symptoms, from the most typical (abdominal pain and distension, flatulence, diarrhea) to many others as nausea, vomiting, headache, poor concentration, severe tiredness, muscular and joint pain, etc.

LactoStrip kit allows the identification of two polymorphisms located at introns 9 (C/T 13910) and 13 (G/A 22018) of MCM6 gene. The presence of those mutations increases the risk of lactose intolerance.

mutaciones relevantes

Detection of the most relevant mutations in one test

distincion homocigosis heterocigosis 1

Distinction of homozygosis and heterozygosis

automatiza proceso hibridacion

Possibility to automate the hybridization process

lectura mediante scanner

Automated reading with scanner available

Our products for the genotyping of polymorphisms associated with lactase persistence

Product
Technique
Sample
Promotional Sheet
CE Decl.
Instruct.
Catalogue No.
LactoStrip
Hybridization on Strip
DNA extracted from blood, saliva or tissues
3.135.016.53.000
It detects the main mutations associated with the persistence or non-persistence of the lactase gene, allowing detection of the possibility of developing lactose intolerance in the future, as well as family studies.

It allows differentiation of whether the mutation is in homozygosis or heterozygosis, which is important for more accurate prediction of the likelihood of developing lactose intolerance.

It is a very easy to interpret test with the help of the standard strip, which can also be carried out with a scanner for greater convenience.

The hybridization step can be automated, which simplifies test performance.

Publications

N.º
Title
Link
Language
C1
Hipolactasia de tipo adulto: polimorfismos C/T-13910 y G/A-22018
Spanish
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information

    Frequently Asked Questions

    The typical profile of a “lactose intolerant patient” is 13910 C/C + 22018 G/G. The typical profile of a lactose tolerant patient is 13910 C/T + 22018 G/A and 13910 T/T + 22018 A/A. In other combinations (heterozygotes + homozygotes), the C/C (intolerant profile) should prevail. In both cases, the patient’s clinical symptoms should be evaluated. If the patient is a carrier of the genotype related to lactose intolerance, and has symptoms related to lactose intolerance, the physician may prescribe a lactose-free diet.

    The LactoStrip test is a tool to assist professionals in the diagnosis of celiac disease, it should be used in conjunction with other tools such as symptoms and the results of other diagnostic tests.
    This test allows genetic lactose intolerance to be discarded if there are no markers. In any case, the symptoms and all the results obtained in the possible complementary tests must be taken into account.
    Yes. Sample can be blood, serum, saliva or any other tissue that contains DNA of the patient. The key is to use the DNA extraction method more appropriate for each kind of sample.
    That is correct. Lactose intolerance is caused by the non persistence of lactasa activity. This means, lactasa activity exists when human is born, so we are tolerant to lactase. When humans grow up, lactase stops being functional for the majority of the global population, so all this people become intolerant to lactose. However, some populations suffered from a genetic mutation that keeps the lactase activity. Nowadays, this is still functional and, in consequence, they are tolerant to lactose.
    Lactose intolerance is not common in infants. In general, it usually appears after the age of three, with the exception of premature birth cases, as those babies are more likely to develop a type of lactose intolerance commonly known as developmental lactase deficiency. This rare disorder involves that the infant cannot break down the lactose in breast milk or formula, and it is usually caused by genes inherited by the parents. Genetic tests indicate the probability of future development of this intolerance, but they are not considered as a diagnostic method by themselves and professionals use them for ruling out the pathology rather than confirming it.