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LactoStrip kit is a test based on the reverse hybridization principle and allows the detection of two polymorphisms of MCM6 gene associated to the lactase persistance in adults: C/T 13910 and G/A 22018.
In most humans, the ability to digest the lactose (the main carbohydrate present in the milk) quickly decreases after the end of lactation period (primary lactose intolerance). This is due to a reduction of the enzyme lactase–phlorizin hydrolase (LPH), responsible for the hydrolysis of lactose to glucose and galactose, sugars that are easily absorbed in the intestine. However, certain individuals, mainly descendants of traditionally cattle-raising peoples, retain the ability to digest lactose in adulthood.
Lactose intolerance presents with diverse symptoms, from the most typical (abdominal pain and distension, flatulence, diarrhea) to many others as nausea, vomiting, headache, poor concentration, severe tiredness, muscular and joint pain, etc.
LactoStrip kit allows the identification of two polymorphisms located at introns 9 (C/T 13910) and 13 (G/A 22018) of MCM6 gene. The presence of those mutations increases the risk of lactose intolerance.
Detection of the most relevant mutations in one test
Distinction of homozygosis and heterozygosis
Possibility to automate the hybridization process
Automated reading with scanner available
The typical profile of a “lactose intolerant patient” is 13910 C/C + 22018 G/G. The typical profile of a lactose tolerant patient is 13910 C/T + 22018 G/A and 13910 T/T + 22018 A/A. In other combinations (heterozygotes + homozygotes), the C/C (intolerant profile) should prevail. In both cases, the patient’s clinical symptoms should be evaluated. If the patient is a carrier of the genotype related to lactose intolerance, and has symptoms related to lactose intolerance, the physician may prescribe a lactose-free diet.
