One-step immunochromatographic test kit for the semi-quantitative detection of human Calprotectin in faecal samples.
Inflammatory bowel disease (IBD), which comprises Crohn’s disease and Ulcerative Colitis, is a severe and incurable chronic disease of the intestinal tract. It affects around 2 million people across Europe, and its prevalence is increasing every day.
The symptoms of IBD closely resemble those of non-inflammatory diseases, such as irritable bowel syndrome. However, untreated IBD can cause serious problems for the patient, so a quick diagnosis to differentiate between the two diseases is crucial in these cases.
Currently, the gold standard technique is endoscopy, which is invasive for the patient, requires anaesthesia and is costly for the practitioner.
Calprotectin is recognised as a potential marker of intestinal mucosal inflammation, and offers a less invasive alternative. It is a hetero-complex with antimicrobial properties that is activated by neutrophils and, once released, can be detected in serum, body fluids and faeces. Several studies show that approximately 90% of patients with colorectal cancer also have increased levels of calprotectin.
Simple and safe
Stool samples can be stored refrigerated (+2-8°C) for up to 7 days. For prolonged storage, samples should be frozen at -20°C. Avoid subjecting the sample to successive freeze/thaw cycles.
As indicated in the instructions for use, it is not recommended to use samples that have been collected in transport media or to which preservation agents (such as formalin, PBS, PVA or similar) or spiking agents have been added, as their presence may interfere with the test.
If symptoms persist, other techniques are recommended. Both celiac disease and microscopic colitis polyps may present with inflammation.
Normal levels of calprotectin in neonates can range from 22 to 860 µg/g, so it is not recommended to use this test in neonatal samples.
In cases such as inflammatory bowel disease, colorectal cancer and other enteropathies, endoscopy and biopsy are also recommended to confirm the diagnosis obtained and to evaluate the degree of inflammation.