The Real Respiratory Bacterial Panel 2 kit, based on the real-time PCR technique, allows the qualitative detection of Bordetella pertussis (BP), Bordetella parapertussis (BPP) and Bordetella holmesii (BH) in samples from the respiratory tract (nasopharyngeal swabs, nasopharyngeal washings, nasopharyngeal aspirates, etc.).
The Bordetella genus is formed of 8 species, of which only the following are human pathogens: B. pertussis, B. parapertussis and B. holmesii. The disease caused by B. pertussis and B. parapertussis is known as whooping cough. It is a major risk today, with 50 million cases per year and 300,000 deaths (mostly among infants and neonates). Therefore, a reliable diagnosis is required to initiate appropriate treatment and contact prophylaxis.
Diagnosis of Bordetellae based on culture or serological techniques has special requirements, is sometimes very time-consuming or does not provide good sensitivity data. For this reason, molecular techniques based on the detection of insertion sequences (IS) can be an interesting alternative to increase the sensitivity in the detection of these pathogens.
The Real Respiratory Bacterial Panel 2 test provides a simple, inexpensive and reliable way to detect all infections caused by bordetellas B. pertussis, B. parapertussis and B. holmesii.
Detection and differentiation of 3 species
Several samples validated
Insertion sequences for greater sensitivity
Internal endogenous control
Results in 30 minutes
No, the test is qualitative. It gives a Ct value, so there is a possibility to compare the values obtained for each sample. Theoretically, the higher the Ct value, the lower the amount of initial DNA, but no numerical correlation can be established.
No. The kit can be transported under refrigerated conditions (2-8°C), maintaining its specifications.
The CFX96 Real-time PCR detection system (BioRad), QuantStudio 5 Real-time PCR system (Applied Biosystems), Gentier 96 Real-time PCR (Tianlong) and Gentier 48 E Real-Time PCR (Tianlong) have been validated for use. The use of other systems must be validated by the user.