Real-time PCR panel for the differential detection of B. pertussis (BP), B. parapertussis (BPP) and B. holmesii (BH)

The Real Respiratory Bacterial Panel 2 kit, based on the real-time PCR technique, allows for the qualitative detection of B. pertussis (BP), B. parapertussis (BPP), and B. holmesii (BH) in samples from the respiratory tract (nasopharyngeal swabs, nasopharyngeal washes, nasopharyngeal aspirates, etc.).

The Real Respiratory Bacterial Panel 2 test is a real-time PCR panel designed for use in laboratories, hospitals and molecular-capable centres, offering an efficient, cost-effective and highly accurate solution for the detection of B. pertussis, B. parapertussis and B. holmesii from respiratory samples (swabs, washings and nasopharyngeal aspirates). Its implementation optimises patient management, reduces diagnostic time and facilitates epidemiological control of pertussis, contributing to better outbreak management and prevention strategies.

The genus Bordetella includes eight species, of which B. pertussis, B. parapertussis and B. holmesii are pathogenic in humans. B. pertussis and B. parapertussis are the main causes of pertussis, a highly contagious respiratory infection that remains a public health problem with approximately 50 million cases annually and 300,000 deaths, mainly in neonates and infants. Given its impact, rapid and accurate diagnosis is essential to initiate appropriate treatment and contact prophylaxis.

Conventional methods, such as bacterial culture or serological tests, have limitations in terms of response time, sensitivity and technical requirements. Therefore, molecular techniques based on real-time PCR have emerged as a highly sensitive and specific alternative, allowing a faster and more reliable identification of these pathogens.

Detection and differentiation of 3 species

Several samples validated

Insertion sequences for greater sensitivity

Internal endogenous control

Results in 30 minutes

Our products for the differential detection of Bordetella pertussis, Bordetella parapertussis y Bordetella homesii

Product	Technique	Sample	Promotional Sheet	CE Decl.	Reference No.
Real Respiratory Bacterial Panel 2	PCR real time	DNA extracted from respiratory tract samples (nasopharyngeal swab/washes/aspirates, exudates)		CE-IVDR	5.182.048.88.000

Feature 1

Several samples validated. The test has been designed and validated for use with DNA obtained from respiratory samples of different nature, which allows the professional to take the sample from one area or another to ensure the capture of as much of the pathogen as possible. Specifically, the kit has been validated with DNA extracted from nasopharyngeal aspirate/wash, nasopharyngeal swab and exudates.

Feature 2

The insertion sequences used for diagnosis are repeated throughout the genome of the bacterium, which allows for more sensitive detection.

Feature 3

The use of internal endogenous controls ensures that the extraction, amplification and subsequent detection are correct.

Feature 4

The kit can be transported refrigerated, thus avoiding the use of dry ice, which makes the process cheaper and easier.

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Frequently Asked Questions

Is the test quantitative?

No, the test is qualitative. It gives a Ct value, so there is a possibility to compare the values obtained for each sample. Theoretically, the higher the Ct value, the lower the amount of initial DNA, but no numerical correlation can be established.

Is dry ice needed to transport the kit?

No. The kit can be transported under refrigerated conditions (2-8°C), maintaining its specifications.

How long does it take to get a result?

The PCR process is completed within 30 minutes.

Which thermal cyclers are validated?

The CFX96 Real-time PCR detection system (BioRad), QuantStudio 5 Real-time PCR system (Applied Biosystems), Gentier 96 Real-time PCR (Tianlong) and Gentier 48 E Real-Time PCR (Tianlong) have been validated for use. The use of other systems must be validated by the user.