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Raw Materials

Large scale production of monoclonal antibodies and recombinant antigens for the immunoassays industry and clinical research.

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RAW MATERIALS

Large scale production of monoclonal antibodies and recombinant antigens for the immunoassays industry and clinical research.

How are our raw materials?

alta calidad

Highest quality

40 anios

More than 40 years of experience

fabricacion

Large-scale manufacturing up to hundreds of grams

paquetes

Optimum batch-to-batch consistency

Monoclonal Antibodies

Antibodies (also called immunoglobulins or Ig) are gamma globulin-type glycoproteins found in soluble form in the blood or other fluids of vertebrates. They act as receptors for B lymphocytes, identifying and neutralising foreign elements such as viruses, bacteria or parasites.

Depending on the heavy chain form of the antibody, there are different isotypes. In mammals, five classes of isotypes (IgG, IgM, IgE, IgA and IgD) have been reported with different functions to direct the necessary immune response depending on the type of foreign bodies they encounter.

Within the antibodies, two types can be differentiated: monoclonal antibodies and polyclonal antibodies. In OPERON, we are dedicated to the manufacture of monoclonal antibodies. These are produced by a cell resulting from the fusion of a B lymphocyte clone and a tumour plasma cell. As all the clones come from a single stem cell, the resulting monoclonal antibodies are identical.

The production of monoclonal antibodies can be carried out in several ways: with tissue cultures whose tumours synthesise the ascitic fluid (fluid with a high concentration of antibodies), or by means of cell culture or bioreaction. In OPERON, the manufacture is always by cell culture or by bioreaction.

By application

Antigen
Clone
Isotype
Purification
Aplication
Other info
Adenovirus
Z-2
IgG2a Kappa
Protein G
IC / ELISA
Against hexon protein (genus specific epitope)
Adenovirus
Z-64
IgG1 Kappa
IEC
IC / ELISA
Against hexon protein (genus specific epitope)
Biotin
U-10
IgG1 Kappa
Protein G
IC / ELISA / BM
Against biotin, used as protein or DNA label
HBsAg (Hepatitis B Virus Surface Antigen)
B-15
IgG1 Kappa
Protein G
IC/ELISA
React with Ad and Ay subtypes
HBsAg (Hepatitis B Virus Surface Antigen)
B-59
IgG2b Kappa
IEC
IC / ELISA
HBsAg (Hepatitis B Virus Surface Antigen)
B-65 Fab´2
IEC/HIC
IC / ELISA
hCG (Human Chorionic gonadotropin)
MH-95
IgG1 Kappa
IEC/HIC
IC / ELISA
hCG conformational.Very good cooperation with MH-109
hCG (Human Chorionic gonadotropin)
MH-95 Fab´2
IEC/HIC
IC / ELISA
hCG conformational.Very good cooperation with MH-109
hCG (Human Chorionic gonadotropin)
MH-109
IgG1 Kappa
IEC/HIC
IC / ELISA
Specific to hCG beta subunit. Very good cooperation with MH-95
Hemocyanin
K-6
IgG1 Kappa
IEC/HIC
IC / ELISA
Antibody against hemocyanin from keyhole limpet (KLH)
Human Hemoglobin
N-12
IgG1 Kappa
IEC/HIC
IC / ELISA
Against human Hemoglobin (recognize A0, A2, S and metahemoglobin). Non cross-reaction with bovine, ovine, pig, pigeon, turkey, rabbit and horse hemoglobin
Human Hemoglobin
N-14
IgG1 Kappa
IEC/HIC
IC / ELISA
Against human Hemoglobin (recognize A0, A2, S and metahemoglobin). Non cross-reaction with bovine, ovine, pig, pigeon, turkey, rabbit and horse hemoglobin
Human IgA
E-24
IgG2b Kappa
IEC/HIC
IC / ELISA
No cross reaction with other human Ig isotypes
PSA (Prostate Specific Antigen)
J-2
IgG1 Kappa
IEC/HIC
IC / ELISA
Recognizes free PSA and PSA-ACT
J-2 Fab´2
IEC/HIC
IC / ELISA
J-12
IgG1 Kappa
Protein G
IC / ELISA
J-12 Fab´2
Protein G
IC / ELISA
Rotavirus
W-2
IgG2a Lambda
IEC
IC / ELISA
Against protein capsid

Antígeno
Clon
Isotipo
Purificación
Aplicación
Otra información
Biotina
U-10
IgG1 Kappa
Proteína G
IC / ELISA / BM
Anticuerpo frente a biotina, utilizada para el marcaje de proteínas y ADN
Digoxigenina
AC-4
IgG1 Kappa
IEC
ELISA / BM
Anticuerpo frente a digoxinenina, utilizada para el marcaje de proteínas y ADN
FITC (isotiocianato de fluoresceína)
Y-7
IgM
GPC
ELISA / BM
Anticuerpo frente a FITC, utilizada para el marcaje de proteínas y ADN
FITC (isotiocianato de fluoresceína)
Fracción IgG de Y-7
GPC
ELISA / BM
Anticuerpo frente a FITC, utilizada para el marcaje de proteínas y ADN
TAMRA
AD-12
IgG1 Kappa
IEC
ELISA / BM
Anticuerpo frente al fluoróforo TAMRA, utilizado para el marcaje de proteínas y ADN

Antigen
Clone
Isotype
Purification
Application
Other info
Adenovirus
Z-2
IgG2a Kappa
Protein G
IC / ELISA
Against hexon protein (genus specific epitope)
Z-64
IgG1 Kappa
IEC
AFP (alpha-fetoprotein)
H-14
IgG1 Kappa
IEC
ELISA
Against human alpha-fetoprotein. No cross reaction with HSA
H-15
IEC/HIC
AFP (alpha-fetoprotein)
C-4
IgG1 Kappa
Protein G
ELISA
Against human alpha-fetoprotein. No cross reaction with HSA
Biotin
U-10
IgG1 Kappa
Protein G
IC / ELISA / BM
Against biotin, used as protein or DNA label
Digoxigenin
AC-4
IgG1 Kappa
IEC
ELISA / BM
Against digoxigenin, used as protein or DNA label
FITC (Fluorescein isothiocyanate)
Y-7
IgM
GPC
ELISA / BM
Against FITC, used as protein or DNA label
IgG Fraction from Y-7
GPC
ELISA / BM
HBsAg (Hepatitis B Virus Surface Antigen)
B-7
IgG1 Kappa
IEC/HIC
ELISA
React with Ad and Ay subtypes.
B-15
IgG1 Kappa
Protein G
IC/ELISA
B-53
IgG2a Kappa
IEC/HIC
ELISA
B-59
IgG2b Kappa
IEC
IC / ELISA
B-65
IgG1 Kappa
IEC/HIC
ELISA
B-65 Fab´2
IEC/HIC
IC / ELISA
hCG (Human Chorionic gonadotropin)
MH-95
IgG1 Kappa
IEC/HIC
IC / ELISA
hCG conformational.Very good cooperation with MH-109
MH-95 Fab´2
MH-109
IgG1 Kappa
Specific to hCG beta subunit. Very good cooperation with MH-95
MH-109 Fab´2
ELISA
Hemocyanin
K-6
IgG1 Kappa
IEC/HIC
IC / ELISA
Antibody against hemocyanin from keyhole limpet (KLH)
HRP (Horseradish Peroxidase)
S-2
IgG1
IEC
ELISA
Against horseradish peroxidase
S-9
Human Hemoglobin
N-12
IgG1 Kappa
IEC/HIC
IC / ELISA
Against human Hemoglobin (recognize A0, A2, S and metahemoglobin). Non cross-reaction with bovine, ovine, pig, pigeon, turkey, rabbit and horse hemoglobin
N-14
Human IgM
E-6
IgG1 Kappa
IEC/HIC
ELISA
No cross reaction with other human Ig isotypes
Human IgG
E-8
Human IgA
E-24
IgG2b Kappa
IC / ELISA
Human IgE
E-27
IgG1 Kappa
IEC
ELISA
No cross reaction with other human Ig isotypes
E-28
IgG1 Kappa
IEC/HIC
E-29
IgG1 Kappa
IEC
p24 HIV (Immunodeficiency virus)
P-2
IgG1 Kappa
IEC/HIC
ELISA
Against denatured and soluble p24 from HIV
P-7
IgG1 Lambda
P-12
IgG1 Kappa
P-16
IgG1 Kappa
P-18
IgG1 Kappa
P-21
IgG1 Kappa
HCV (Hepatitis C virus)
O-6
IgG1 Kappa
IEC/HIC
ELISA
Against core protein from HCV
PSA (Prostate Specific Antigen)
J-2
IgG1 Kappa
IEC/HIC
IC / ELISA
Recognizes free PSA and PSA-ACT
PSA (antígeno prostático específico)
J-2 Fab´2
IEC/HIC
J-12
IgG1 Kappa
Protein G
J-12 Fab´2
Protein G
Rabbit Ig´s
F-4
IEC
ELISA
Against rabbit immnoglobulins
F-7
Respiratory Syncytial Virus
AE-15
IgM Kappa
GPC
ELISA
RSV strain: long
AE-17
IgG1 Kappa
IEX
Rotavirus
W-2
IgG2a Lambda
IEC
IC / ELISA
Against protein capsid
TAMRA
AD-12
IgG1 Kappa
IEC
ELISA / BM
Against TAMRA, used as protein or DNA label

Recombinant Antigens

Antigens are all substances that can be recognised by the adaptive immune system, whether their own or foreign. Normally, they are mainly proteins or polysaccharides: i.e. parts of bacteria (capsule, cell wall, flagella, fimbriae, and toxins), viruses or other micro-organisms.

So what is the difference between an antigen and an immunogen? An immunogen always induces a protective immune response when it is introduced into the body. On the other hand, an antigen is a molecule that is capable of binding to an antibody, but does not necessarily induce an immune response.

Recombinant antigens are produced using the recombinant DNA technique. This technique is based on expressing a gene from one organism in a different organism, such as bacteria, yeast, insect or mammalian cells.

By joining DNA sequences from two different organisms that are not normally found together, an artificial DNA molecule (recombinant DNA) can be produced in vitro. In an organism, the recombinant DNA causes a genetic modification by the addition of a new DNA sequence. This, at the same time, leads to the expression of new traits or modification of existing ones.

By use

Recombinant antigens
Organism
Source
Specification
Application
tTG Recombinant human tissue transglutaminase
Human
Escherichia Coli
Native recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 80.328 Dalton.Calculated isoelectric point: 5.2 Useful for immunoassay. (Application: immunochromatography and ELISA.)
IC/ELISA
MACH Chimeric CHAGAS multiantigen
Trypanosoma cruzi
Chimeric recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 9.9 Dalton. Calculated isoelectric point: 8.12. Useful for immunoassay. (Application: immunochromatography and ELISA.)
1F8 CHAGAS antigen
Trypanosoma cruzi
Recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 25.845 Dalton. Calculated isoelectric point: 5.39. Useful for immunoassay. (Application: immunochromatography and ELISA.)
gp41 Envelope protein HIV virus type 1
Human Immunodeficiency Virus 1
Recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 18.692 Dalton. Calculated isoelectric point: 7.47 No cross reaction with positive sera to HBsAg and HCV. Useful for immunoassay. (Application: immunochromatography and ELISA.)
gp36 Envelope protein HIV virus type 2
Human Immunodeficiency Virus 2
Recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 20.775 Dalton. Calculated isoelectric point: 8.25 No cross reaction with positive sera to HBsAg and HCV. Useful for immunoassay. (Application: immunochromatography and ELISA.)

Recombinant antigens
Organism
Source
Specification
Application
tTG Recombinant human tissue transglutaminase
Human
Escherichia Coli
Native recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 80.328 Dalton.Calculated isoelectric point: 5.2 Useful for immunoassay. (Application: immunochromatography and ELISA.)
IC/ELISA
MACH Chimeric CHAGAS multiantigen
Trypanosoma cruzi
Chimeric recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 9.9 Dalton. Calculated isoelectric point: 8.12. Useful for immunoassay. (Application: immunochromatography and ELISA.)
1F8 CHAGAS antigen
Trypanosoma cruzi
Recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 25.845 Dalton. Calculated isoelectric point: 5.39. Useful for immunoassay. (Application: immunochromatography and ELISA.)
gp41 Envelope protein HIV virus type 1
Human Immunodeficiency Virus 1
Recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 18.692 Dalton. Calculated isoelectric point: 7.47 No cross reaction with positive sera to HBsAg and HCV. Useful for immunoassay. (Application: immunochromatography and ELISA.)
gp36 Envelope protein HIV virus type 2
Human Immunodeficiency Virus 2
Recombinant protein from fermentor culture. Purified by affinity chromatography. Molecular weight: 20.775 Dalton. Calculated isoelectric point: 8.25 No cross reaction with positive sera to HBsAg and HCV. Useful for immunoassay. (Application: immunochromatography and ELISA.)

Features

At OPERON we have bioreactors to manufacture monoclonal antibodies on a large scale, up to hundreds of grams, making us the perfect partner for leading IVD manufacturers.

We offer monoclonal antibodies of the highest quality, uniform and with optimal inter-batch consistency, thanks to strict quality controls and the extensive experience of our R&D team. In fact, we have been developing hybridomas since 1978.

Many of the antibodies and recombinant antigens marketed by OPERON are routinely used to produce our own IVD tests (hCG, HBsAg, Rotavirus, PSA, Chagas, Celiac disease, …).

Purification and characterisation. Combination of several chromatographic techniques: AIEX, HIC and protein A/G/L. Optimal customer service and technical support.

Labelling: peroxidase, phosphatase, biotin, latex particles, colloidal gold,… Additional customised services for our customers’ projects and immunoassays.

Serum-free culture media. IgG and IgM fragmentation. Antibody engineering for optimisation of antibody properties. Technically excellent solutions.

Contact us for more
information

    FAQ

    OPERON manufactures the antibodies and recombinant antigens included in its catalogue on a large scale (up to hundreds of grams), depending on demand of each product. In addition, we offer a tailor-made service to manufacture monoclonal antibodies in large scale using customer’s hybridomas. These hybridomas are received at our facilities and we agree with the customer the quantities and type of processes, purification, formats, etc., optimal for the manufacture. For large-scale production of recombinant antigens, the transforming cells required for production are received and, as in the previous case, the terms of supply and manufacturing processes are agreed with the customer.

    OPERON supplies monoclonal antibodies with a concentration of 5-10 mg/ml, purity >80 % by AIEX, HIC, GPC, Protein A/G/L or CIEX , in PBS, Borate, Carbonate or Tris-HCl buffer. Recombinant antigens are provided at >90 % purity by affinity chromatography, at a concentration of 1-2 mg/ml in Tris or HEPES buffer.

    OPERON produces only monoclonal antibodies. We do not manufacture polyclonal antibodies.
    OPERON uses Escherichia coli (E. coli) bacteria for the production of recombinant proteins. This results in a higher specific growth rate, easier genetic manipulation, no expensive culture media requirements and a wide range of stable expression vectors.