Infections of S. pneumoniae represent an important public health problem in both industrialized nations as well as those that are less-developed. It is responsible for high mortality and lethality, as it is one of the main causative agents for a large variety of clinical conditions, from benign infections like otitis media and acute sinusitis to more severe infections such as septicaemia, meningitis and pneumonia. In fact, 1,6 million deaths are registered each year due to invasive pneumococcal disease. From all of them, 800,000 cases are children under 5 years.
The WHO recommends periodical surveys in all the countries for monitorizing the vaccine impact and for evaluating vaccination strategies. Therefore, S. pneumoniae serotyping is of particular importance from a clinical and epidemiological point of view.
In general, this serotyping is performed using a technique known as Quellung, which is considered nowadays as the Gold Standard. Nevertheless, the use of this method is time-consuming, elaborate and it requires specialized staff. In fact, its use is restricted to qualified laboratories.
S. PneumoStrip kit is the only test in the market that allows the easy and rapid identification of 76 serotypes S. pneumoniae, including the 23 used in the vaccines available (7-valent, 10-valent, 13-valent and 23-valent).
Only commercial molecular test
Perfect correlation with Gold Standard
76 serotypes in one test
Possibility to automate the hybridization process
No need of previous culture
The kit was initially designed to be used on culture samples, so the best results are obtained using them. Nevertheless, it has been shown in different studies to be useful also with non-cultured samples. In those cases, the sensitivity of the test may be affected by having less DNA in the starting sample, which would be reflected in the lack of signal in the internal controls of the test. If so, sensitivity can be increased by performing individual PCRs with each set of primers, instead of performing a single PCR with all primers.
No. The detection of 3 internal controls allows unequivocal identification of S. pneumoniae, even in the presence of other pneumococci.